Foundational Research
Introduction
標的分子の鋳型をポリマーで取る技術、分子インプリンティングは、天然の抗体と同様、標的分子を選択的に認識可能な、分子インプリントポリマー（MIP）を得る方法として知られ、MIPは人工抗体とも呼ばれる。安価・安定に大量生産できることから、高価で不安定な生体材料に替わる材料として注目されている。私達は、このMIPをさらに高機能化するための新しい戦略、インプリンティング後にさらに化学修飾を施すポストインプリンティング修飾（PIM）を提案している。これは、生体で行われているタンパク質が生合成されたのちに化学修飾されて高機能タンパク質を生み出す、翻訳「後」修飾（PTM）と同様に、分子の鋳型を取った後、さらに機能性分子でPIMを行うことで、結合特性の調整、結合情報の可視化、分子認識能のスイッチングなど、従来法では達成できなかった多機能化を達成する。認識対象は多岐にわたり、抗生物質や環境ホルモンからタンパク質、細胞外小胞エクソソーム、細胞にいたる様々な物質に適用可能である。
TearExo Method
TearExo法に用いられるセンシングチップの大きな特徴は、涙液中の細胞外小胞を見分けるために、「生体由来抗体と人工高分子受容体を空間的に統合したハイブリッド認識界面」を備えている点にある。まず、分子インプリンティングにより、細胞外小胞の形や表面の性質に合う人工のくぼみをポリマー材料の表面につくる。このくぼみは、細胞外小胞と同様のサイズに調製可能である。
さらに、PIMにより、このくぼみの中だけに、生体由来の抗体と蛍光分子を選択的に配置する。これにより、くぼみ自体が細胞外小胞を形や表面性質でとらえ、くぼみ内部に固定された抗体が特定の表面マーカーを確認し、同じ空間に置かれた蛍光分子が結合を信号として知らせる、という一連の働きを一つの認識空間で実現できる。
従来の抗体だけを使う方法に比べて、人工材料による安定性や設計自由度を活かせる一方、人工材料だけでは難しい高い分子識別性を抗体によって補える点が独創的である。つまりTearExo法のチップは、人工材料の丈夫さと抗体の正確な見分ける力を、標的が入る"くぼみ"の中に集約したハイブリッド型センサーであり、微量の涙液から細胞外小胞を高感度に検出するための技術である。
Protein Imprinting
がんを検知・診断するためのバイオマーカータンパク質を高感度･高選択的に認識・検出するための人工高分子材料を分子インプリンティングとPIMの技術を用いて開発した。
Oriented Imprinting
従来の分子インプリンティングは、重合中、鋳型分子が自由運動するため、取れる鋳型の均一性が低かった。鋳型分子を単に固定化して自由運動を低減するだけではなく、配向固定化することで、鋳型の均一性が格段に向上する。配向固定化とPIMを組み合わせることにより、従来のMIPに比べ、高い機能性をもつMIPが創製可能である。
Small Molecule Imprinting
環境ホルモンや抗生物質などの生物活性低分子化合物を高感度･高選択的に認識・検出するための人工高分子材料を分子インプリンティングとPIMの技術を用いて開発した。
MIP Array
複数のMIPを用い、それぞれのMIPに対する結合特性を多変量解析することで、標的タンパク質をフィンガープリント化して区別することが可能であった。従来のプロテインチップに代わる保存安定性が高く安価なプロテイン解析法として期待される。
Nanomedicine
ナノ材料は、次世代医療のためのナノメディシンとして期待されている。特定のタンパク質を認識する分子インプリントナノ材料を用いて高機能性薬物送達システムを創製し、従来困難であった難治性疾患のためのナノメディシンの開発を開発する。本研究は、材料の設計合成から動物実験に至る広範囲な知識と技術が必要なことから、東京大学、神戸大学、大阪市立大学と協力して研究を遂行している。
SPR Sensing
プラズモニックチップ(波長オーダーの周期構造をもつ金属薄膜被覆センサチップ)を用いた増強蛍光によるバイオマーカー超高感度センシングデバイスの開発を関西学院大学と協力して行っている。また、局在表面プラズモン共鳴とMIPあるいは特異リガンドをもつポリマーナノ粒子を合成し、共鳴吸収波長のシフトを利用したバイオマーカーの検出を行った。
MIP Nanoparticle Sensing
ストレスマーカーのコルチゾールを認識するMIPナノ粒子を設計･合成し、蛍光偏光解消法により高感度にコルチゾールのセンシングを行った。
Automated Analysis
カスタムメイドで顕微鏡を装着した自動分注蛍光計測装置を開発し、抗体、DNA、MIPなどが固定化された微小反応板が挿入可能な特殊扁平ピペットチップを用いて、全自動バイオ分析システムを構築した。この業績により、株式会社TearExo CTOの高野が、2025年日本分析化学会女性アナリスト賞を授与された。
Publications
[1] Sunayama, H., Matsui, Y., Takeuchi, T. Functional Molecule-Integrated Nano-Interfaces for Detection of Extracellular Vesicles, Adv. Sensor Res. 2025, 4, 2400186. (DOI: 10.1002/adsr.202400186)
[2] Sunayama, H, Cheubong, C, Takano, E., Takeuchi, T., Facile biotic/abiotic sandwich detection system for the highly sensitive detection of human serum albumin and glycated albumin, Anal. Bioanal. Chem. 2024, 416, 7337-7345. (DOI: 10.1007/s00216-024-05403-9)
[3] Fukutomi, K., Fujimoto, E., Shimokawatoko, M., Takano, E., Sunayama, S., Takeuchi, T., Tawa, K. Single-Extracellular-Vesicle Detection with a Plasmonic Chip and Enhanced Fluorescence Microscopy, ACS Omega 2024, 9, 44396-44406. (DOI: 10.1021/acsomega.4c05678)
[4] Cheubong, C., Sunayama, H., Takano, E., Kitayama, Y., Minami, H., Takeuchi, T. A Rapid Abiotic/Biotic Hybrid Sandwich Detection System for Trace Pork Adulteration in Halal Meat Extract, Nanoscale 2023, 15, 15171-15178. (DOI: 10.1039/d3nr02863a)
[5] Mizutani, K., Kanda, M., Sunayama, H., Takeuchi, T., Tawa, K., New plasmonic sensor platform using a spatially controlled photochemical reaction and enhanced fluorescence, J. Photochem. Photobiol. A Chem. 2022, 433, 114177. (DOI: 10.1016/J.JPHOTOCHEM.2022.114177)
[6] Kitayama, Y., Yamada, T., Kiguchi, K., Yoshida, A., Hayashi, S., Akasaka, H., Igarashi, K., Nishimura, Y., Matsumoto, Y., Sasaki, R., Takano, E., Sunayama, H., Takeuchi, T. In vivo stealthified molecularly imprinted polymer nanogels incorporated with gold nanoparticles for radiation therapy, J. Mater. Chem. B 2022, 10, 6784-6791. (DOI: 10.1039/D2TB00481J)
[7] Oshita, A, Sunayama, H., Takeuchi, T. A molecularly imprinted nanocavity with transformable domains that fluorescently indicate the presence of antibiotics in meat extract samples, J. Mater. Chem. B 2022, 10, 6682-6687. (DOI: 10.1039/d2tb00145d)
[8] Hayakawa, N., Kitayama, Y., Igarashi, K., Matsumoto, Y., Takano, E., Sunayama, H., Takeuchi, T. Fc domain-imprinted stealth nanogels capable of orientational control of immunoglobulin G adsorbed in vivo, ACS Applied Mater. Interfaces 2022, 14, 16074-16081. (DOI: 10.1021/acsami.2c01953)
[9] Yoshida, A., Kitayama, Y., Hayakawa, N., Mizukawa, Y., Nishimura, Y., Takano, E., Sunayama, H., Takeuchi, T. Biocompatible polymer-modified gold nanocomposites of different shapes as radiation sensitizers, Biomater. Sci. 2022, 10, 2665-2672. (DOI: 10.1039/D2BM00174H)
[10] Cheubong, C., Takano, E., Kitayama, Y., Sunayama, H., Minamoto, K., Takeuchi, R., Furutani, S., Takeuchi, T., Molecularly imprinted polymer nanogel-based fluorescence sensing of pork contamination in halal meat extracts, Biosens. Bioelectron. 2021, 172, 112775. (DOI: 10.1016/j.bios.2020.112775)
[11] Tsutsumi, K., Sunayama, H., Kitayama, H., Takano, E., Nakamachi, Y., Sasaki, R., Takeuchi, T., Fluorescent signaling of molecularly imprinted nanogels prepared via postimprinting modifications for specific protein detection, Adv. NanoBiomed Res. 2021, 1, 2000079. (DOI: 10.1002/anbr.202000079)
[12] Sunayama, H., Takamiya, K., Takano, E. Horikawa, R., Kitayama, Y., Takeuchi, T., Simultaneous detection of two tumor marker proteins using dual-colored signaling molecularly imprinted polymers prepared via multi-step post-Imprinting modifications, Bull. Chem. Soc. Jpn. 2021, 94, 525-531. (DOI: 10.1246/bcsj.20200254)
[13] Sunayama, H., Takeuchi, T., Protein-imprinted polymer films prepared via cavity-selective multi-step post-imprinting modifications for highly selective protein recognition, Anal. Bioanal. Chem. 2021, 413, 6183-6189. (DOI: 10.1007/S00216-021-03386-5)
[14] Ikegami, T., Kataoka, R., Sunayama, H., Takeuchi, T., In silico characterization of binding properties on a molecularly imprinted polymer, BUNSEKIKAGAKU 2021, 70, 111-124. (DOI: 10.2116/BUNSEKIKAGAKU.70.111)
[15] Morishita, T., Yoshida, A., Hayakawa, N., Kiguchi, K., Cheubong, C., Sunayama, H., Kitayama, Y., Takeuchi, T., Molecularly imprinted nanogels possessing dansylamide interaction sites for controlling protein corona in situ by cloaking intrinsic human serum albumin, Langmuir 2020, 36, 10674-10682. (DOI: 10.1021/acs.langmuir.0c00927)
[16] Kitayama, Y., Takeuchi, T., Photodegradable polymer capsules fabricated via interfacial photocross-linking of spherical polymer particles, ACS Appl. Polym. Mater. 2020, 2, 3813-3820. (DOI: 10.1021/acsapm.0c00472)
[17] Saeki, T., Takano, E., Sunayama, H., Kamon, Y., Horikawa, R., Kitayama, Y., Takeuchi, T., Signalling molecular recognition nanocavities with multiple functional groups prepared by molecular imprinting and sequential post-imprinting modifications for prostate cancer biomarker glycoprotein detection, J. Mater. Chem. B 2020, 8, 7987-7993. (DOI: 10.1039/D0TB00685H)
[18] Inubushi, S., Kawaguchi, H., Mizumoto, S., Kunihisa, T., Baba, M., Kitayama, Y., Takeuchi, T., Hoffman, R. M., Sasaki, R., Oncogenic miRNAs identified in tear exosomes from metastatic breast cancer patients, Anticancer Res. 2020, 40, 3091-3096. (DOI: 10.21873/anticanres.14290)
[19] Takeuchi, T., Mori, K., Sunayama, H., Takano, E., Kitayama, Y., Shimizu, T., Hirose, Y., Inubushi, S., Sasaki, R., Tanino, H. Antibody-conjugated signaling nanocavities fabricated by dynamic molding for detecting cancers using small extracellular vesicle markers from tears, J. Am. Chem. Soc. 2020, 142, 6617-6624. (DOI: 10.1021/jacs.9b13874)
[20] Cheubong, C., Yoshida, A., Mizukawa, Y., Hayakawa, N., Takai, M., Morishita, T., Kitayama, Y., Sunayama, H., Takeuchi, T. Molecularly imprinted nanogels capable of porcine serum albumin detection in raw meat extract for halal food control, Anal. Chem. 2020, 92, 6401-6407. (DOI: 10.1021/acs.analchem.9b05499)
[21] Hayakawa, N., Yamada, T., Kitayama, Y., Takeuchi, T., Cellular interaction regulation by protein corona control of molecularly imprinted polymer nanogels using intrinsic proteins, ACS Appl. Polym. Mater. 2020, 2, 1465-1473. (DOI: 10.1021/acsapm.9b01149)
[22] Uchiyamada, K., Okubo, K., Asakawa, K., Kamon, Y., Kitayama, Y., Takeuchi, T., Suzuki, H. Perforated bimodal interferometric biosensor for affinity sensing, Adv. Mater. Technol. 2019, 4, 1800533. (DOI: 10.1002/admt.201800533)
[23] Yoshida, A.; Kitayama, Y.; Kiguchi, K.; Yamada, T.; Akasaka, H.; Sasaki, R.; Takeuchi, T. Gold nanoparticle-incorporated molecularly imprinted microgels as radiation sensitizers in pancreatic cancer, ACS Appl. Bio Mater. 2019, 2, 1177-1183. (DOI: 10.1021/acsabm.8b00766)
[24] Matsumoto, H., Sunayama, H., Kitayama, Y., Takano, E., Takeuchi, T. Site-specific post-imprinting modification of molecularly imprinted polymer nanocavities with a modifiable functional monomer for prostate cancer biomarker recognition, Sci. Technol. Adv. Mater. 2019, 20, 305-312. (DOI: 10.1080/14686996.2019.1583495)
[25] Mori, K., Hirase, M., Morishige, T., Takano, E., Sunayama, H., Kitayama, Y., Inubushi, S., Sasaki, R., Yashiro, M., Takeuchi, T. A pretreatment-free, polymer-based platform prepared by molecular imprinting and post-imprinting modifications for sensing intact exosomes, Angew. Chem. Int. Ed. 2019, 58 (6), 1612-1615. (DOI: 10.1002/anie.201811142)
[26] Morishige, T., Takano, E., Sunayama, H., Kitayama, Y., Takeuchi, T. Post-imprinting-modified molecularly imprinted nanocavities with two synergetic, orthogonal, glycoprotein-binding sites to transduce binding events into fluorescence changes, ChemNanoMat 2019, 5 (2), 224-229.
[27] Saeki, T., Sunayama, H., Kitayama Y., Takeuchi, T. Orientationally fabricated zwitterionic molecularly imprinted nanocavities for highly sensitive glycoprotein recognition, Langmuir 2019, 35, 5, 1320-1326.
[28] Kitayama, Y., Takeuchi, T. Morphology control of shell-crosslinked polymer particles fabricated by photo-induced shell-selective crosslinking approach via dispersed state control, J. Colloid Interface Sci. 2018, 530, 88-97.
[29] Ichikawa, S., Shimokawa, N., Takagi, M., Kitayama, Y., Takeuchi, T., Size-dependent uptake of electrically neutral amphipathic polymeric nanoparticles by cell-sized liposomes and an insight into their internalization mechanism in living cells, Chem. Commun. 2018, 54, 4557-4560.
[30] Kamon, Y., Takeuchi, T. Molecularly imprinted nanocavities capable of ligand-binding domain and size/shape recognition for selective discrimination of vascular endothelial growth factor isoforms, ACS Sens. 2018, 3 (3), 580-586.
[31] Ooya, T., Ogawa, T., Takeuchi, T. Temperature-induced recovery of a bioactive enzyme using polyglycerol dendrimers: correlation between bound water and protein interaction, J. Biomater. Sci., Polym. Ed. 2018, 29 (6), 701-715.
[32] Uchiyamada, K., Okubo, K., Asakawa, Y., Kamon, Y., Kitayama, Y., Takeuchi, T., Suzuki, H. Directional coupler biosensor with molecularly imprinted polymer, Sens. Mater. 2018, 30 (5), 1009-1017.
[33] Kitayama, Y., Yoshikawa, K., Takeuchi, T. Post-Crosslinked Molecular Imprinting with Functional Polymers as a Universal Building Block for Artificial Polymeric Receptors, Macromolecules 2017, 50 (19), 7526-7534.
[34] Suda, N., Sunayama, H., Kitayama, Y., Kamon, Y., Takeuchi, T. Oriented, Molecularly Imprinted Cavities with Dual Binding Sites for Highly Sensitive and Selective Recognition of Cortisol, R. Soc. Open Sci. 2017, 4, 170300. (DOI: 10.1098/rsos.170300)
[35] Kitayama, Y., Takeuchi, T. Fabrication of Redox-responsive Degradable Capsule Particles via Shell-Selective Photo-induced Crosslinking Approach from Spherical Polymer Particles, Chem. Eur. J. 2017, 23, 12870-12875.
[36] Takeuchi, T., Kitayama, Y, Sasao, R., Yamada, T., Toh, K., Matsumoto, Y., Kataoka, K. Molecularly Imprinted Nanogels Acquire Stealth in situ by Cloaking Themselves with Native Dysopsonic Proteins, Angew. Chem. Int. Ed. 2017, 56, 7088-7092. [Hot Paper]
[37] Sunayama, H., Kitayama, Y., Takeuchi, T. Regulation of Protein Binding Activities of Molecularly Imprinted Polymers via Post-imprinting Modifications to Exchange Functional Groups within the Imprinted Cavity, J. Mol. Recogn. 2017, 31, e2633.
[38] Ohmori, K., Ooya, T., Takeuchi, T. Crosslinked Network with Rotatable Binding Sites Based on Mono-carboxylated a-Cyclodextrin [2]Rotaxane Capable of Angiotensin III Recognition, Chem. Eur. J. 2017, 23 (19), 4708-4712.
[39] Nakai, S.; Sunayama, H.; Kitayama, Y.; Nishijima, M.; Wada, T.; Inoue, Y. Takeuchi, T. A Regioselective Molecularly Imprinted Reaction Field for the [4+4] Photocyclodimerization of 2-Anthracenecarboxylic Acid, Langmuir 2017, 33, (9), 2103-2108.
[40] Sunayama, H., Ohta, T., Kuwahara, A., Takeuchi, T. Fluorescent Signaling Molecularly Imprinted Polymers for Antibiotics Prepared via Site-Directed Post-Imprinting Introduction of Plural Fluorescent Reporters within the Recognition Cavity, J. Mater. Chem. B 2016, 4, 7138-7145.
[41] Sunayama, H., Ohta, T., Kuwahara, A., Takeuchi, T. Fluorescent Signaling Molecularly Imprinted Polymers for Antibiotics Prepared via Site-Directed Post-Imprinting Introduction of Plural Fluorescent Reporters within the Recognition Cavity, J. Mater. Chem. B 2016, 4, 7138-7145.
[42] Horikawa, R., Sunayama, H., Kitayama, Y. Takano, E., Takeuchi, T. Programmable signaling molecular recognition nano-cavity prepared by molecular imprinting and post-imprinting modifications, Angew. Chem. Int. Ed. 2016, 55, 13023-13027.
[43] Kitayama, Y., Yoshikawa, K., Takeuchi, T. An efficient pathway for preparing hollow particles: site-specific crosslinking of spherical polymer particles with photo-responsive groups that play a dual role in shell crosslinking and core shielding, Langmuir 2016, 32, 9245-9253.
[44] Kamon, Y., Inoue, Mihara, E., Kitayama, Y., Ooya, T., Takeuchi, T. Hydrophilic crosslinked-polymeric surface capable of effective suppression of protein adsorption, Appl. Surf. Sci. 2016, 378, 467-472.
[45] Murase, N., Mukawa, T., Sunayama, H., Takeuchi, T. Molecularly imprinted polymers bearing spiropyran-based photoresponsive binding sites capable of photo-triggered switching for molecular recognition activity, J. Polym. Sci. B: Polym. Phys. 2016, 54, (16) 1637-1644.
[46] Murase, N., Taniguchi, S., Takano, E., Kitayama, Y., Takeuchi, T. Molecularly imprinted nanocavity-based fluorescence polarization assay platform for cortisol sensing, J. Mater. Chem. B 2016, 4, 1770-1777.
[47] Matsuura, R,, Tawa, K., Kitayama, Y., Takeuchi, T. A plasmonic chip-based bio/chemical hybrid sensing system for the highly sensitive detection of C-reactive protein, Chem. Commun. 2016, 52, 3883-3886.
[48] Yoshizawa, S., Kuwata, T., Takano, E., Kitayama, Y., Takeuchi, T. Transcription-type protein imprinted polymers for SPR sensing prepared using target-immobilized stamps based on submicrometer-sized particles via biotin-avidin linkage, Molecular Imprinting 2016, 3, 26-34.
[49] Moriishi, M., Kitayama, Y., Ooya, T., Takeuchi, T. Amphiphilic polymerizable porphyrins conjugated to a polyglycerol dendron moiety as functional surfactants for multifunctional polymer particles, Langmuir 2015, 31, 12903-12910.
[50] Kuwata, T., Uchida, A., Takano, E., Kitayama, Y., Takeuchi, T. Molecularly imprinted polymer arrays as synthetic protein chips prepared by transcription-type molecular imprinting by use of protein-immobilized dots as stamps, Anal. Chem. 2015, 87, 11784-11791.
[51] Takimoto, K., Takano, E., Kitayama, Y., Takeuchi, T. Synthesis of monodispersed submillimeter-sized molecularly imprinted particles selective for human serum albumin using inverse suspension polymerization in water-in-oil emulsion prepared using microfluidics, Langmuir 2015, 31, 4981-4987.
[52] Murase, N., Taniguchi, S., Takano, E., Kitayama, Y., Takeuchi, T. Fluorescence reporting of binding interaction of target molecules with core-shell type cortisol imprinted polymer particles using environmentally responsible fluorescent-labeled cortisol, Macromol. Chem. Phys. 2015, 216, 1396-1404.
[53] Kamon, Y., Kitayama, Y., Itakura, A. N., Fukazawa, K., Ishihara, K., Takeuchi, T. Synthesis of grafted phosphorylcholine polymer layers as specific recognition ligands for C-Reactive protein focused on grafting density and thickness to achieve highly sensitive detection, Phys. Chem. Chem. Phys. 2015, 17, 9951-9958.
[54] Taguchi, H., Sunayama, H., Takano, E., Kitayama, Y., Takeuchi, T. Preparation of Molecularly imprinted polymers for the recognition of proteins via the generation of peptide-fragment binding sites by semi-covalent imprinting and enzymatic digestion, Analyst 2015, 140, 1448-1452.
[55] Sunayama, H., Takeuchi, T. Molecularly imprinted protein recognition cavities bearing exchangeable binding sites for postimprinting site-directed introduction of reporter molecules for readout of binding events, ACS Appl. Mater. Interfaces 2014, 6, 20003-20009.
[56] Kitamura, A., Kitayama, Y., Ooya, T., Takeuchi, T. Molecularly Imprinted Recognition Polymers for Catechin Recognition Prepared Using Dummy-Template Molecules, Chromatography 2014, 35, 139-145.
[57] Kitamura, A., Kitayama, Y., Ooya, T., Takeuchi, T. Molecularly imprinted polymers for catechin recognition prepared using dummy-template molecules, Chromatography 2014, 35, 139-145.
[58] Takeuchi, T., Mori, T., Kuwahara, A., Ohta, T., Oshita, A., Sunayama, H., Kitayama Y., Ooya, T. Conjugated protein mimics with molecularly imprinted reconstructible and transformable regions assembled using space-filling prosthetic groups, Angew. Chem. Int. Ed. 2014, 53, 12765-12770.
[59] Sasaki, S., Kitayama, Y., Ooya, T., Takeuchi, T. Molecularly imprinted protein recognition thin films constructed by controlled/living radical polymerization, J. Biosci. Bioeng. 2015, 119, 200-205.
[60] Kamon, Y., Matsuura, R., Kitayama, Y., Ooya, T., Takeuchi, T.. Precisely controlled molecular imprinting of glutathione-s-transferase by orientated template immobilization using specific interaction with an anchored ligand on a gold substrate, Polym. Chem. 2014, 5, 4764-4771.
[61] Kitayama, Y., Takeuchi, T.. Localized surface plasmon resonance nanosensing of C-reactive protein with poly(2-methacryloyloxyethyl phosphorylcholine)-grafted-gold nanoparticles prepared by surface-initiated atom transfer radical polymerization, Anal. Chem. 2014, 86, 5587-5594.
[62] Sunayama, H., Ooya, T., Takeuchi, T. Fluorescent protein-imprinted polymers capable of signal transduction of specific binding events prepared by a site-directed two-step postimprinting modification, Chem. Commun. 2014, 50, 1347-1349.
[63] Uchida, A., Kitayama, Y., Takano, E., Ooya, T., Takeuchi, T. Supraparticles comprised of molecularly imprinted nanoparticles and modified gold nanoparticles as a nanosensor platform, Rsc Advances 2013, 3, 25306-25311.
[64] Inoue, N., Ooya, T., Takeuchi, T. Hydrophilic molecularly imprinted polymers for bisphenol A prepared in aqueous solution, Microchim. Acta 2013, 180, 15, 1387-1392.
[65] Suga, Y., Sunayama , H., Ooya, T., Takeuchi, T. Molecularly imprinted polymers prepared using protein-conjugated cleavable monomers followed by site-specific post-imprinting introduction of fluorescent reporter molecules, Chem. Commun. 2013, 49, 8450-8452.
[66] Inoue, Y., Kuwahara, A., Ohmori, K., Sunayama, H., Ooya, T., Takeuchi, T. Fluorescent Molecularly Imprinted Polymer Thin Films for Specific Protein Detection Prepared with Dansyl Ethylenediamine-Conjugated O-Acryloyl L-Hydroxyproline Biosens. Bioelectron. 2013, 48, 113-119.
[67] Taguchi, Y., Takano, E., Takeuchi, T. SPR sensing of bisphenol A using molecularly imprinted nanoparticles immobilized on slab optical waveguide with consecutive parallel Au and Ag deposition bands coexistent with Bisphenol A-immobilized Au nanoparticles, Langmuir 2012, 28, 7083-7088.
[68] Takano, E., Tanaka, F., Ooya, T., Takeuchi, T. Molecularly Imprinted Microspheres for Bisphenol A Prepared Using a Microfluidic Device, Anal. Sci. 2012, 28, 457-462.
[69] Takano, E., Taguchi, Y., Ooya, T., Takeuchi, T. Dummy template-imprinted polymers for Bisphenol A prepared using a Schiff base-type template molecule with post-imprinting oxidation, Anal. Lett. 2012, 45, 1204-1213.
[70] Inoue, J., Ooya, T., Takeuchi, T. Protein imprinted TiO2-coated quantum dots for fluorescent protein sensing prepared by liquid phase deposition, Soft Matter 2011, 7, 9681-9684.
[71] Sasaki, S., Ooya, T., Takeuchi, T. Highly Selective Bisphenol A-Imprinted Polymers Prepared by Atom Transfer Radical Polymerization, Polym. Chem. 2010, 1, 1684-1688.
[72] Sunayama, H., Ooya, T., Takeuchi, T., Fluorescent Protein Recognition Polymer Thin Films Capable of Selective Signal Transduction of Target Binding Events Prepared by Molecular Imprinting with a Post-Imprinting Treatment, Biosens. Bioelectron. 2010, 26, 458.462.
[73] Takeda, K., Kuwahara, A., Ohmori K., Takeuchi, T. Molecularly Imprinted Tunable Binding Sites Based on Conjugated Prosthetic Groups and Ion-Paired Cofactors, J. Am. Chem. Soc. 2009, 131, 8833-8838.
[74] Shinmori, H., Furukawa, H., Fujimoto, K., Shimizu, H., Inouye, M., Takeuchi, T. Characteristic Fluorescence Behavior of Dialkynylpyrene Derivatives in Hydrophobic Cavity of Protein, Chem. Lett. 2009, 38, 84-85.
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